{"created":"2023-05-15T10:54:54.499607+00:00","id":4641,"links":{},"metadata":{"_buckets":{"deposit":"a3caa972-0941-4192-a238-242831a1b103"},"_deposit":{"created_by":17,"id":"4641","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"4641"},"status":"published"},"_oai":{"id":"oai:juen.repo.nii.ac.jp:00004641","sets":[]},"author_link":["14211","14212","14213","14214"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"299","bibliographicPageStart":"293","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"上越教育大学研究紀要"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Laser microdissection and subsequent direct DNA amplification methods are both effective for plant genome research. By using these techniques, we isolated barley chromosome 6 and amplified the target gene, a-amyl. Barley a-amyl gene, encoding the high-pI a-amylase, has been mapped onto the long arm of chromosome 6 by Southern hybridization and isozyme analysis using lines with specific chromosome constitution. The primers based on the a-amyl sequence were used for PCR with the isolated chromosomes. When the five microisolated chromosome 6 were used as the template, expected fragments with 142 bp were amplified. By contrast, no PCR product was detected when five chromosome 7 were used as a negative control. As a result, we directly confirmed that the a-amyl gene locates on barley chromosome 6, and concluded that five microisolated chromosomes are sufficient for direct amplification of the target gene.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"上越教育大学"}]},"item_10002_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"AN10284863","subitem_relation_type_select":"NAID"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-8162","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mitsunaga,  Shinichiro"}],"nameIdentifiers":[{"nameIdentifier":"14211","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamaguchi,  Junji"}],"nameIdentifiers":[{"nameIdentifier":"14212","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohyama,  Hideo"}],"nameIdentifiers":[{"nameIdentifier":"14213","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukui,  Kiichi"}],"nameIdentifiers":[{"nameIdentifier":"14214","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-06-17"}],"displaytype":"detail","filename":"kiyo22_2-01.pdf","filesize":[{"value":"374.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kiyo22_2-01.pdf","url":"https://juen.repo.nii.ac.jp/record/4641/files/kiyo22_2-01.pdf"},"version_id":"5ae72850-a472-4114-b006-20cfe34a415a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"direct amplification","subitem_subject_scheme":"Other"},{"subitem_subject":"α-Amylase gene","subitem_subject_scheme":"Other"},{"subitem_subject":"barley","subitem_subject_scheme":"Other"},{"subitem_subject":"microdissection method","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Direct Amplification of an α-Amylase Gene from Barley Chromosome 6 by a Microdissection Method","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Direct Amplification of an α-Amylase Gene from Barley Chromosome 6 by a Microdissection Method","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"17","path":["1001"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-05-22"},"publish_date":"2009-05-22","publish_status":"0","recid":"4641","relation_version_is_last":true,"title":["Direct Amplification of an α-Amylase Gene from Barley Chromosome 6 by a Microdissection Method"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2024-03-11T05:23:54.754404+00:00"}