{"created":"2023-05-15T10:55:01.967804+00:00","id":4796,"links":{},"metadata":{"_buckets":{"deposit":"804e6db7-9ec1-4de9-9d15-6ba8fcbeec3f"},"_deposit":{"created_by":17,"id":"4796","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"4796"},"status":"published"},"_oai":{"id":"oai:juen.repo.nii.ac.jp:00004796","sets":[]},"author_link":["14665","14666","14667","14668","14669","14670"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"751","bibliographicPageStart":"741","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"上越教育大学研究紀要"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"クリの緑葉からゲノムDNAの単離を試みた。DNAの純度を高めるために，抽出に用いる界面活性剤，酸化防止剤，水溶性ポリマ，ポリアミンなどについて検討を加えた。その結果，セチルトリメチルアンモニウムブロミド（CTAB）とポリビニルピロリドン（PVP）を用いた方法，ドデシル硫酸ナトリウム（SDS）とPVPを用いた方法，および多糖類除去のための樹脂を用いた方法によりゲノムDNAの単離に成功した。このようにして得られたゲノムDNAは，制限酵素反応やポリメラーゼ連鎖反応（PCR）に利用することが可能であった。","subitem_description_type":"Abstract"},{"subitem_description":"We have developed the genomic DNA isolation procedure for the chestnut leaf. The factors affecting yield, such as detergents, reductants, water-soluble polymers, and polyamines are discussed. Isolated DNA is suitable for the restriction enzyme reaction and polymerase chain reaction (PCR).","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"上越教育大学"}]},"item_10002_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"AN10284863","subitem_relation_type_select":"NAID"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-8162","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"光永,  伸一郎"},{"creatorName":"ミツナガ, シンイチロウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"14665","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"宮村,  祥代"},{"creatorName":"ミヤムラ, サチヨ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"14666","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"下村,  正彦"},{"creatorName":"シモムラ, マサヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"14667","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mitsunaga,  Shin-ichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"14668","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyamura,  Sachiyo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"14669","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimomura,  Masahiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"14670","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-06-17"}],"displaytype":"detail","filename":"kiyo23_2-29.pdf","filesize":[{"value":"570.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kiyo23_2-29.pdf","url":"https://juen.repo.nii.ac.jp/record/4796/files/kiyo23_2-29.pdf"},"version_id":"21e5b24a-58ec-427e-b9ba-4d9025ee064b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"クリ","subitem_subject_scheme":"Other"},{"subitem_subject":"ゲノムDNA","subitem_subject_scheme":"Other"},{"subitem_subject":"単離","subitem_subject_scheme":"Other"},{"subitem_subject":"Chestnut","subitem_subject_scheme":"Other"},{"subitem_subject":"Genomic DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"Isolation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"クリ緑葉からのゲノムDNAの単離","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"クリ緑葉からのゲノムDNAの単離","subitem_title_language":"ja"}]},"item_type_id":"10002","owner":"17","path":["1006"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-05-25"},"publish_date":"2009-05-25","publish_status":"0","recid":"4796","relation_version_is_last":true,"title":["クリ緑葉からのゲノムDNAの単離"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2024-03-11T05:27:35.645522+00:00"}