| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2009-05-22 |
| タイトル |
|
|
タイトル |
Direct Amplification of an α-Amylase Gene from Barley Chromosome 6 by a Microdissection Method |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
direct amplification |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
α-Amylase gene |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
barley |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
microdissection method |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者 |
Mitsunaga, Shinichiro
Yamaguchi, Junji
Ohyama, Hideo
Fukui, Kiichi
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Laser microdissection and subsequent direct DNA amplification methods are both effective for plant genome research. By using these techniques, we isolated barley chromosome 6 and amplified the target gene, a-amyl. Barley a-amyl gene, encoding the high-pI a-amylase, has been mapped onto the long arm of chromosome 6 by Southern hybridization and isozyme analysis using lines with specific chromosome constitution. The primers based on the a-amyl sequence were used for PCR with the isolated chromosomes. When the five microisolated chromosome 6 were used as the template, expected fragments with 142 bp were amplified. By contrast, no PCR product was detected when five chromosome 7 were used as a negative control. As a result, we directly confirmed that the a-amyl gene locates on barley chromosome 6, and concluded that five microisolated chromosomes are sufficient for direct amplification of the target gene. |
| 書誌情報 |
上越教育大学研究紀要
巻 22,
号 2,
p. 293-299,
発行日 2003-03
|
| 出版者 |
|
|
出版者 |
上越教育大学 |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0915-8162 |
| 論文ID（NAID） |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
NAID |
|
|
関連識別子 |
AN10284863 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
kiyo22_2-01.pdf (374.2 kB)
